Analysis of antimicrobial activity of Ashurak extract prepared with different solvents on Klebsiella pneumoniae and Shigella dysentery isolated from poultry feces

Abstract

Bacteria are more likely to cause disease than foodborne pathogens. Articles have shown that most plant extracts have antimicrobial properties. The aim of this article was to analyze the antimicrobial activity of Ashurak extract prepared with different solvents on K. pneumoniae and Sh. dysentery isolated from poultry feces. In this article, antimicrobial effects of various extracts (ethyl acetate, aqueous, ethanolic, methanolic and hydroalcoholic) on K. pneumoniae bacteria Sh. dysentery isolated from poultry feces was obtained by diffusion method in Müller Hinton agar medium using 6 mm paper discs according to the instructions. Mean comparison was performed using two-way analysis of variance and post hoc test with the least significant difference. The lowest inhibitory concentrations of aqueous, ethanolic, methanolic and hydroalcoholic extracts of Ashurak on K. pneumoniae were 12.5, 12.5, 12.5 and 3.1 ppm, respectively. The lowest inhibitory concentration of aqueous, ethanolic, methanolic and hydroalcoholic extracts of Ashurak on SchiegelDysentery was equal to 12.5, 12.5, 25 and 25 ppm. Among the mentioned extracts, the aqueous extract had the greatest effect on the inhibition of K. pneumoniae , but there was no difference between the extracts. According to the results of the present study and the increasing resistance to synthetic antibacterial substances, it seems that Ashurak can be considered as effective plants in cleansing some bacteria, including K. pneumoniae and Sh. dysentery . In addition, the results of this study showed that Ashurak plant is more effective In contrast; K. pneumoniae had dysentery compared to Shigella.