A high throughputs and consistent method for the sampling and isolation of Endophytic bacteria allied to high altitude the medicinal plant Arnebia benthamii (Wall ex. G. Don)

Abstract

Bacterial communities in the root zones are vibrant and play an essential role in medicinal plant microbe interaction and are a major driving force for maintaining the stability of soil ecosystem. The major bottleneck for the studying the interaction lies on sampling methods. Though various methods from time to time have been reported and developed however each method exhibited variation and inconsistency. We recommend here the standardized method after consulting the major developed methods so far across the globe. Primarily we divide the root zone in major bulk and rhizopshere fractions and a definite bacterial gradient around the root zone of the medicinal plant Arnebia benthamii (Wall ex. G. Don), a high altitude medicinal herb was observed. We propose to use this method as general basis for soil sampling and enumeration of bacterial diversity from root systems