Phytochemical Analysis and Antibacterial Activity of the Kenyan Wild Orchids

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Published

2021-11-21

DOI:

https://doi.org/10.54458/mev.v1i02.6677

Keywords:

Qualitative, infections, extracts, compounds, management

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Issue

Vol. 1 No. 02 (2021): Micro Environer

Section

Research Article

Authors

  • Polycarp Chagona Department of Pure and Applied sciences, Technical University of Mombasa, P.O Box 90420 – 80100, Mombasa, Kenya
  • Polycarp Chagona Department of Pure and Applied sciences, Technical University of Mombasa, P.O Box 90420 – 80100, Mombasa, Kenya
  • Nancy Kwamboka Nairobi Botanic Garden Section, National Museums of Kenya (NMK), P.O. Box 40658- 00100, Nairobi, Kenya
  • Humphrey Gaya Kenya Forestry Research Institute (KEFRI), P.O. Box 20412-00200, Nairobi, Kenya.
  • Huxley Makonde Department of Pure and Applied sciences, Technical University of Mombasa, P.O Box 90420 – 80100, Mombasa, Kenya
  • Aggrey Adem Department of Mathematics and Physics, Technical University of Mombasa, P.O Box 90420 – 80100, Mombasa, Kenya
  • Kenneth Osano Department of Biochemistry, Uzima University, P.O. Box 2502-40100, Kisumu, Kenya
  • Fanuel Kawaka Department of Biological Sciences, Jaramogi Oginga Odinga University of Science and Technology, P.O. Box 210 – 40601, Bondo, Kenya

Abstract

The current study evaluated the antibacterial activity of dichloromethane and methanol (DCM-MeOH) extracts of four Kenyan orchid species against three selected bacterial strains. Extracts of E1 (Ansellia Africana), E2 (Trydactylescottelli), E3 (Polystachyabella) and E4 (Liparis bowkeri) were screened for antibacterial activity against staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginosa using agar disc diffusion. Ampicillin was included as a positive control. Qualitative analysis revealed the presence of flavonoids, saponins, alkaloids, tannins, terpenoids, steroids and glycosides. Trydactyle scottelli and Polystachyabella extracts revealed a more substantial presence of tannins and steroids, respectively, compared to others. All extracts showed varying levels of antibacterial activity against the test bacteria. However, Polystachyabella and Liparis bowkeri against Bacillus subtilis and Ansellia Africana against Pseudomonas aeruginosa exhibited higher activities similar to that of Ampicillin. The study further showed that the DCM-MeOH extracts of the four orchids contain potential compounds that should be screened for conventional management of bacterial infections.

 

 

How to Cite

Chagona, P., Chagona, P., Kwamboka, N., Humphrey Gaya, Makonde, H., Adem, A., Osano, K., & Kawaka, F. (2021). Phytochemical Analysis and Antibacterial Activity of the Kenyan Wild Orchids. Micro Environer, 1(02), 93–100. https://doi.org/10.54458/mev.v1i02.6677

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Author Biography

Polycarp Chagona, Department of Pure and Applied sciences, Technical University of Mombasa, P.O Box 90420 – 80100, Mombasa, Kenya

 

 

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